CP02 | Genetically engineered mouse models for the study of cell death and its role in immunity and inflammation
This central service project aims to facilitate access to already existing genetic mouse models that are relevant for cell death research, and provides service for the generation of new genetically modified mice for the work within the SFB. Within CP02 a central repository of genetic mouse models is available that provides both live animals but also cells from these mice to the CRC projects.
You can find more information about the mouse repository here or at this sciebo folder (you can get the password from the SFB1403 office).
Prof Dr. Branko Zevnik
CECAD in vivo Research Facility, University of Cologne
Contact: branko.zevnik(at)uk-koeln(dot)de
For more information visit: CECAD ivRF
Prof. Dr. Manolis Pasparakis
Institute for genetics - CECAD - University of Cologne
Contact: pasparakis(at)uni-koeln(dot)de
For more information visit: Pasparakis lab
Project related Publications
2011 - 2019
Tröder, S.E., Ebert, L.K., Butt, L., Assenmacher, S., Schermer, B., and Zevnik, B. (2018). An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes. PLoS One 13, e0196891.
Taraborrelli, L., Peltzer, N., Montinaro, A., … Pasparakis, M., …, and Walczak, H. (2018). LUBAC prevents lethal dermatitis by inhibiting cell death induced by TNF, TRAIL and CD95L. Nat Commun 9, 3910.
Lin, J., Kumari, S., Kim, C., Van, T.M., Wachsmuth, L., Polykratis, A., and Pasparakis, M. (2016). RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation. Nature 540, 124-128.
Vlantis, K., Wullaert, A., Polykratis, A., Kondylis, V., Dannappel, M., Schwarzer, R., Welz, P., Corona, T., Walczak, H., Weih, F., Klein, U., Kelliher, M. and Pasparakis, M. (2016). NEMO Prevents RIP Kinase 1-Mediated Epithelial Cell Death and Chronic Intestinal Inflammation by NF-kappaB-Dependent and -Independent Functions. Immunity 44, 553-567.
Kondylis, V., Polykratis, A., Ehlken, H., Ochoa-Callejero, L., Straub, B.K., Krishna-Subramanian, S., Van, T.M., Curth, H.M., Heise, N., Weih, F., Klein, U., Schirmacher, P., Kelliher, M. and Pasparakis, M. (2015). NEMO Prevents Steatohepatitis and Hepatocellular Carcinoma by Inhibiting RIPK1 Kinase Activity-Mediated Hepatocyte Apoptosis. Cancer Cell 28, 582-598.
Dannappel, M., Vlantis, K., Kumari, S., Polykratis, A., Kim, C., Wachsmuth, L., Eftychi, C., Lin, J., Corona, T., Hermance, N., Zelic, M., Kirsch, P., Basic, M., Bleich, A., Kelliher, M. & Pasparakis, M. (2014). RIPK1 maintains epithelial homeostasis by inhibiting apoptosis and necroptosis. Nature 513, 90-94.
Bonnet, M.C., Preukschat, D., Welz, P.S., van Loo, G., Ermolaeva, M.A., Bloch, W., Haase, I., and Pasparakis, M. (2011). The adaptor protein FADD protects epidermal keratinocytes from necroptosis in vivo and prevents skin inflammation. Immunity 35, 572-582.
Welz, P.S., Wullaert, A., Vlantis, K., Kondylis, V., Fernandez-Majada, V., Ermolaeva, M., Kirsch, P., Sterner-Kock, A., van Loo, G., and Pasparakis, M. (2011). FADD prevents RIP3-mediated epithelial cell necrosis and chronic intestinal inflammation. Nature 477, 330-334.