B08 | Connecting plant sensor NLRs to host cell death responses
Prof. Dr. Jijie Chai
Department of Biochemistry, University of Cologne
Dr. Paul Schulze-Lefert
Department Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research
Contact: schlef(at)mpipz.mpg(dot)de
For more information visit: Schulze-Lefert Lab
This project was funded within the SFB 1403 from 2020 - 2023
Former Abstract
Nucleotide-binding domain/leucine-rich repeat (NLR) receptors provide a crucial barrier to pathogen infection in mammals and plants. In both systems, NLRs sense microbe-mediated perturbations inside host cells and, via conformational switches, induce cell death and anti-microbial pathways leading to disease resistance. This project integrates protein structure-function studies with in vivo molecular and pathology phenotyping, genetics and cell biology to answer fundamental questions about how plant sensor NLRs activate cell death pathways and whether different NLR types converge on the same or distinct cell death machineries in immunity.
Recent Publications
Project A08 Publications 1st Funding Period 2020 - 2023
2022
Kožich, V., Schwahn, B.C., Sokolová, J., Křížková, M., Ditroi, T., Krijtm J., Khalil, Y., Křížek. T., Vaculíková-Fantlová, T., Stibůrková, B., Milla, P., Clayton, P., Barvíková, K., Blessing, H., Sykut-Cegielska, J., Dionisi-Vici, C., Gasperini, A., García-Cazorla, A., Haack, T.B., Honzík, T., Ješina, P., Kuster, A., Laugwitz, L., Martinelli, D., Porta, F., Santer, R., Schwarz, G., Nagy, P. (2022) Human ultrarare genetic disorders of sulfur metabolism demonstrate redundancies in H2S homeostasis. Redox Biology 58 (2022) 102517 https://doi.org/10.1016/j.redox.2022.102517
2021
Kaczmarek, A.T., Bender, D., Gehling, T., Kohl, J.B., Daimagüler, H.S., Santamaria-Araujo, J.A., Liebau, M.C., Koy, A., Cirak, S., Schwarz, G. (2021) A defect in molybdenum cofactor binding causes an attenuated form of sulfite oxidase deficiency. JMD https://doi.org/10.1002/jimd.12454
Mellis, A-T., Misko, A.L., Arjune, S., Liang, Y., Erdélyi, K., Ditrói, T., Kaczmarek, A.T., Nagy, P., Schwarz, G. (2021) The role of glutamate oxaloacetate transaminases in sulfite biosynthesis and H2S metabolism.Redox Biology Volume 38 2021, https://doi.org/10.1016/j.redox.2020.101800
Project related Publications
2009 - 2019
Belaidi, A.A., and Schwarz, G. (2013). Molybdenum cofactor deficiency: metabolic link between taurine and S-sulfocysteine. Adv Exp Med Biol 776, 13-19.
Bender, D., Kaczmarek, A.T., Niks, D., Hille, R., and Schwarz, G. (2019). Mechanism of nitrite-dependent NO synthesis by human sulfite oxidase. Biochem J. 476, 1805-1815
Bender, D., Kaczmarek, A.T., Santamaria-Araujo, J.A., Stueve, B., Waltz, S., Bartsch, D., Kurian, L., Cirak, S., and Schwarz, G. (2019b). Impaired mitochondrial maturation of sulfite oxidase in a patient with severe sulfite oxidase deficiency. Hum Mol Genet. in press, pii: ddz109
Frohlich, M., Dejanovic, B., Kashkar, H., Schwarz, G., and Nussberger, S. (2014). S-palmitoylation represents a novel mechanism regulating the mitochondrial targeting of BAX and initiation of apoptosis. Cell Death Dis 5, e1057.
Kaczmarek, A.T., Strampraad, M.J.F., Hagedoorn, P.L., and Schwarz, G. (2019). Reciprocal regulation of sulfite oxidation and nitrite reduction by mitochondrial sulfite oxidase. Nitric Oxide 89, 22-31.
Kohl, J.B., Mellis, A.T., and Schwarz, G. (2019). Homeostatic impact of sulfite and hydrogen sulfide on cysteine catabolism. Br J Pharmacol 176, 554-570.
Kumar, A., Dejanovic, B., Hetsch, F., Semtner, M., Fusca, D., Arjune, S., Santamaria-Araujo, J.A., Winkelmann, A., Ayton, S., Bush, A.I., et al.Schwarz, G.*, and Belaidi, A.A.(2017). S-sulfocysteine/NMDA receptor-dependent signaling underlies neurodegeneration in molybdenum cofactor deficiency. J Clin Invest 127, 4365-4378.
Schwahn, B.C., Van Spronsen, F.J., Belaidi, A.A., Bowhay, S., Christodoulou, J., Derks, T.G., Hennermann, J.B., Jameson, E., Konig, K., McGregor, T.L., et al.Schwarz, G. (2015). Efficacy and safety of cyclic pyranopterin monophosphate substitution in severe molybdenum cofactor deficiency type A: a prospective cohort study. Lancet 386, 1955-1963.
Schwarz, G., Mendel, R.R., and Ribbe, M.W. (2009). Molybdenum cofactors, enzymes and pathways. Nature 460, 839-847
Veldman, A., Santamaria-Araujo, J.A., Sollazzo, S., Pitt, J., Gianello, R., Yaplito-Lee, J., Wong, F., Ramsden, C.A., Reiss, J., Cook, I., et al.Schwarz, G. (2010). Successful treatment of molybdenum cofactor deficiency type A with cPMP. Pediatrics 125, e1249-1254