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A02 – Cross-talk between dying and bystander cells in vitro and in vivo: an optogenetics approach

Ana J. Garcia-Saez

Institute for genetics, CECAD, University of Cologne
Contact: garcia-office(at)uni-koeln.de
For more information see: Garcia-Saez lab

Manolis Pasparakis

Institute for genetics, CECAD, University of Cologne
Contact: pasparakis(at)uni-koeln.de
For more information see: Pasparakis lab

Abstract

Currently available tools do not allow studying the cross-talk between dying and bystander cells in a spatially and temporally resolved manner. The project aims to develop optogenetically regulated systems allowing the precisely controlled induction of different forms of cell death at a single cell level and use them to study the cross-talk between dying and bystander cells in vitro and in vivo. By employing selective light-controlled activation of different death effectors, this project will study how the different types of cell death shape tissue responses and the underlying mechanisms.

Recent Publications

2023

Czabotar, P.E. and Garcia-Saez, A.J. (2023) Mechanisms of BCL-2 family proteins in mitochondrial apoptosis. Nat Rev Mol Cell Biol. doi: 10.1038/s41580-023-00629-4


Rieger, J., Fitz, M., Fischer, S.M., Wallmeroth, N., Flores-Romero, H., Fischer, N.M., Brand, L.H., García-Sáez, A.J., Berendzen, K.W. and Mira-Rodado, V. (2023) Exploring the Binding Affinity of the ARR2 GARP DNA Binding Domain via Comparative Methods. Genes (Basel). doi: 10.3390/genes14081638


Röck, B.F., Shalaby, R. and García-Sáez, A.J. (2023) Pyroptosis Induction and Visualization at the Single-Cell Level Using Optogenetics. Methods Mol Biol. 2696, 135-147. doi: 10.1007/978-1-0716-3350-2_10 (review)


Pedrera, L., Ros, U. and García-Sáez, A.J. (2023) Calcium as a master regulator of ferroptosis and other types of regulated necrosis. Cell Calcuim 114, 102778 . doi: https://doi.org/10.1016/j.ceca.2023.102778


Shalaby, R., Diwan, A., Flores-Romero, H., Hertlein, V., and Garcia Saez, A.J. (2023). Visualization of BOK pores independent of BAX and BAK reveals a similar mechanism with differing regulation. Cell Death Differ 30, 731–741 https://doi.org/10.1038/s41418-022-01078-w


2022

Cosentino, K., Hertlein, V., Jenner, A., Dellmann, T., Gojkovic, M., Peña-Blanco, A., Dadsena, S., Wajngarten, N., Danial, J.S.H., Thevathasan, J.V., Mund, M., Ries, J., Garcia-Saez, A.J. (2022) The interplay between BAX and BAK tunes apoptotic pore growth to control mitochondrial-DNA-mediated inflammation. Molecular Cell 82, 1-17, February 17, 2022 https://doi.org/10.1016/j.molcel.2022.01.008

2021

Flores-Romero, H., Hohorst, L., John, M., Albert, M.-C., King, L.E., Beckmann, L., Szabo, T., Hertlein, V., Luo, X., Villunger, A., Frenzel, L.P., Kashkar, H., & Garcia-Saez, A.J. (2021) BCL-2-family protein tBID can act as a BAX-like effector of apoptosis. The EMBO Journal (2021) e108690 DOI10.15252/embj.2021108690

2020

Pedrera, L., Espiritu, R., A., Ros, U., Weber, J., Schmitt, A., Stroh, J., Hailfinger, S., von Karstedt, S., García-Sáez, A., J. (2020) Ferroptotic pores induce Ca 2+ fluxes and ESCRT-III activation to modulate cell death kinetics. Cell Death Differ Dec 17. doi: 10.1038/s41418-020-00691-x


Flores‐Romero, H., Ros, U., Garcia-Saez, A.J. (2020) Pore formation in regulated cell death. EMBO J (2020)e105753 https://doi.org/10.15252/embj.2020105753

Project related Publications