CP04 - Proteomic dissection of cell death-mediated protein release and proteolysis
Pitter Huesgen
Institute of Biology II, University of Freiburg
Contact: pitter.huesgen(at)biologie.uni-freiburg.de
For more information visit: Huesgen lab
Felix Meissner
Institute for innate immunity, University Bonn
Contact: felix.meissner(at)uni-bonn.de
For more information visit: Meissner lab
Abstract
This project is funded within the 2nd funding period of the SFB 1403, starting from 2024
This core project aims to i) develops tailored mass spectrometry-based approaches for the comprehensive analysis of cell death released proteins and protease substrates in both mammalian and plant systems and ii) advise all interested CRC members on experimental design and execution of proteomics experiments, including sample preparation, measurement and initial data analysis, and contribute to data interpretation and dissemination. Thus, this core project will provide essential tools and expertise to decipher the mechanisms underlying the cross-talk between dying and bystander cells.
Recent Publications
Weiss FD, Alvarez Y, Shakeri F, Sahu A, Leka P, Dernst A, Rollheiser J, Vasconcelos M, Geraci A, Duthie F, Stahl R, Lee HE, Gellner AK, Buness A, Latz E, Meissner F. Retention of ES cell-derived 129S genome drives NLRP1 hypersensitivity and transcriptional deregulation in Nlrp3tm1Flv mice. Cell Death Differ. 2024 Sep 17. doi: 10.1038/s41418-024-01379-2. Epub ahead of print. PMID: 39289506.
Mooney BC, Doorly CM, Mantz M, García P, Huesgen PF, Graciet E. Hypoxia represses pattern-triggered immune responses in Arabidopsis. Plant Physiol. 2024 Aug 19:kiae432. doi: 10.1093/plphys/kiae432. Epub ahead of print. PMID: 39158089.
van Midden KP, Mantz M, Fonovič M, Gazvoda M, Svete J, Huesgen PF, van der Hoorn RAL, Klemenčič M. Mechanistic insights into CrCEP1: A dual-function cysteine protease with endo- and transpeptidase activity. Int J Biol Macromol. 2024 Jun;271(Pt 1):132505. doi: 10.1016/j.ijbiomac.2024.132505. Epub 2024 May 18. PMID: 38768911.